Figure 2.
The cellular on-rate for PT-VWD platelets is higher than that of healthy platelets. Microspheres coated with recombinant VWF-A1 protein at a concentration that did not support translocation were perfused over a confluent monolayer of surface-immobilized healthy or PT-VWD platelets at wall shear rates ranging from 20 to 300 s-1. The frequency of tether formation was determined by quantifying the number of beads that transiently attached to the substrate over 1 minute and subsequently dividing by the measured flux of beads near the wall over the same time period.9 Data represent the mean ± SD of 4 independent experiments.
