The effect of shear force on the kinetics and mechanical strength of mutant tether bonds. Cellular dissociation rate constants were obtained for VWF-A1–coated beads interacting with a healthy or PT-VWD platelet substrates. The k_off results are plotted as a function of the calculated force on the tether bond, F_b (72.41 × wall shear stress).⁹  The experimental data were fit to the Bell equation based on Monte Carlo (MC) and statistical point estimate (SPE) analyses. Table shows values for k_off as a function of applied force. Data represent the mean ± SD for 5 independent experiments at each wall shear stress tested. *P < .05.