Figure 4.
Figure 4. ATRA-induced down-regulation of cyclins A, B, D3, and E is inhibited by expression of Stat1Ser727Ala or Stat1Tyr701Phe. (A) U-937 sublines were induced by ATRA for 72 hours, and total mRNA was prepared. The cyclin mRNA levels after ATRA treatment were analyzed by RPA using the hCYC-1 multitemplate probe. The lower panels show quantitation of cyclin mRNA expression that was found to be appreciably changed by ATRA treatment, normalized to GAPDH (fold induction). Mean ± SD (n = 3-4). (B-C) The protein levels of cyclins A, B, D2, and D3 in pCIneo, Stat1.2, Tyr701Phe.8, and Ser727Ala.4 cells were determined after 72 hours of ATRA stimulation by Western blot analysis (B), and the level of cyclin E was analyzed after 48 hours of treatment (C). (D) Expression of cyclins A, B, D3, and E in Ser727Ala.5 cells (C) were determined after 72 hours of ATRA stimulation by Western blot analysis.

ATRA-induced down-regulation of cyclins A, B, D3, and E is inhibited by expression of Stat1Ser727Ala or Stat1Tyr701Phe. (A) U-937 sublines were induced by ATRA for 72 hours, and total mRNA was prepared. The cyclin mRNA levels after ATRA treatment were analyzed by RPA using the hCYC-1 multitemplate probe. The lower panels show quantitation of cyclin mRNA expression that was found to be appreciably changed by ATRA treatment, normalized to GAPDH (fold induction). Mean ± SD (n = 3-4). (B-C) The protein levels of cyclins A, B, D2, and D3 in pCIneo, Stat1.2, Tyr701Phe.8, and Ser727Ala.4 cells were determined after 72 hours of ATRA stimulation by Western blot analysis (B), and the level of cyclin E was analyzed after 48 hours of treatment (C). (D) Expression of cyclins A, B, D3, and E in Ser727Ala.5 cells (C) were determined after 72 hours of ATRA stimulation by Western blot analysis.

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