Figure 2.
IFN-γ expression profiles from cultured NK cells in response to 6-hour cytokine stimulation from WT and Stat6-/- mice. (A) Cultured NK cells were stimulated with the indicated cytokines for 6 hours and the culture supernatant analyzed for IFN-γ production. White bars (□) indicate WT mice; black bars (▪), Stat6-/- mice. (B) IFN-γ mRNA expression from the same experiment with L32 as a loading control. (C) Densitometric analysis of panel B. White bars (□) indicate WT mice; black bars (▪), Stat6-/- mice. The data are representative of more than 3 separate experiments. NS indicates nonstimulated.