PCR amplification of promoter/enhancer elements of the vectors generated. To determine that all vectors were generated, 293T and MM1 cells were transduced with these vectors, expanded, and total genomic DNA extracted. The presence of lentivector sequences was determined by PCR using primers common to all the vectors generated (Figure 1). Plasmids used to generate the vectors were used as controls. To ensure that equal amounts of genomic DNA were used, 2 PCR primers that amplify a region of the human dystrophin gene were also included in the reaction mixture. The expected product is 230 bp in length. The PCR products were run on a 1% agarose gel and stained with ethidium bromide. The data shown are for 293T cells.