Analysis of SDF-1, VCAM-1, and β1 integrin expression in normal human CD34+ cells and effects of anti—IL-15 mAb. Flow cytometry analysis shows the effect of overnight treatment with neutralizing anti—IL-15 mAb on the cytoplasmic expression of SDF-1, VCAM-1, and β1 integrin in BM (A) and CB (B) CD34⁺ cells. In CB progenitors, SDF-1 expression was also analyzed after cell treatment with the specific NF-κB inhibitor BAY 11-7082. Each figure is representative of at least 3 different experiments. Vertical bars indicate negative control intensity. Thin lines indicate basal expression, whereas solid bold lines indicate protein expression in treated cells.