Human lymphocytes cultured in the presence of IL-2, IL-7, or IL-15 in the absence of TCR triggering are efficiently transduced by lentiviral vectors. PBMCs isolated from healthy donors were cultured in media alone or stimulated with 10 U/mL IL-2, 5 ng/mL IL-7, 10 ng/mL IL-15, or activated with 30 ng/mL OKT3 and 1 μg/mL anti-CD28, and cultured with 50 U/mL IL-2. (A) Cells were incubated for 3 hours with LV at different MOI: 1 × 10⁶, 5 × 10⁶, 2 × 10⁷ TU/mL 96 hours after culture was started. (B) Cells were transduced with the highest vector dose (2 × 10⁷ TU/mL) after 48, 72, or 96 hours of culture. Data shown are from 1 representative experiment of 3.