Figure 3.
Figure 3. Effect of ectopic overexpression of Bcl-2 or Bcl-xL on 17-AAG—induced apoptosis in MB-468 cells. Ectopic overexpression of Bcl-2 or Bcl-xL in MB-468 cells confers resistance against 17-AAG—induced apoptosis. MB-468 (control 468 cells) and cells stably transfected with Bcl-2 (468/Bcl-2) or Bcl-xL (468/Bcl-xL) cells were exposed to 0.1, 0.25, and 0.50 μM 17-AAG for 48 or 96 hours. Following this, untreated and treated cells were stained with annexin V. Positively stained apoptotic cells were quantitated by flow cytometry. Values represent the mean ± SE of 3 independent experiments.

Effect of ectopic overexpression of Bcl-2 or Bcl-xLon 17-AAG—induced apoptosis in MB-468 cells. Ectopic overexpression of Bcl-2 or Bcl-xL in MB-468 cells confers resistance against 17-AAG—induced apoptosis. MB-468 (control 468 cells) and cells stably transfected with Bcl-2 (468/Bcl-2) or Bcl-xL (468/Bcl-xL) cells were exposed to 0.1, 0.25, and 0.50 μM 17-AAG for 48 or 96 hours. Following this, untreated and treated cells were stained with annexin V. Positively stained apoptotic cells were quantitated by flow cytometry. Values represent the mean ± SE of 3 independent experiments.

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