Kinetics of 17-AAG—mediated depletion of c-Raf-1, S-Src, Akt, S6K1, and PARP cleavage activity of caspase-3. HL-60/Neo (A) and HL-60/Bcl-2 (B) cells were treated with 2.0 × 17-AAG for up to 32 hours. Following the indicated time intervals, cells were harvested and Western blot analyses of Akt, c-Src, c-Raf-1, and S6K1 as well as PARP were performed. As the loading control, β-actin was used.