Effect of ectopic expression of Bcl-2 on 17-AAG—mediated depletion of motochondrial and cytosolic fractions of c-Raf-1. Ectopic expression of Bcl-2 does not affect 17-AAG—mediated depletion of motochondrial and cytosolic fractions of c-Raf-1. HL-60/Neo (A) and HL-60/Bcl-2 (B) cells were treated with 2.0 μM 17-AAG for the indicated exposure intervals. Following this, S100 and heavy-membrane fractions (HMFs) were obtained from harvested cells and used for immunoblot analysis of c-Raf-1 by means of anti—c-Raf-1 antibody. As the loading control, β-actin was used.