Figure 7.
High and comparable levels of hematopoietic progenitors in Lin-Fas- and Lin-Fas+ cells. CD34+ CB (A) or BM (B) cells were cultured in SF medium supplemented with SFT3 for 5 days and subsequently sorted into Lin-Fas- (□) and Lin-Fas+ (▪) cells (as shown in Figure 5). Both populations were plated at one cell per well in SF medium supplemented with SFT3 (“Materials and methods”). Plates were scored for total clonal growth and clonal size (from 3 cells to 100% of well covered by cells) as indicated. Each group consisted of 120 wells, with a maximum of 76 clones expected per group (“Materials and methods”). Results represent a total of 3 independent experiments (2 CB and 1 BM). A zero indicates no clones observed.