Figure 1.
Figure 1. Angiotensin II enhances O2- and ROS production in a time- and dose-dependent manner in human neutrophils. Inhibitory effect of irbesartan. Neutrophils were suspended in KR-HEPES buffer at 106 cells/mL in the presence of 15 μM lucigenin (A,C) or 15 μM luminol (B,D). Reactions were started by the addition of angiotensin II (Ang) at the indicated concentrations. The effect of irbesartan (Irb, 10 μg/mL) on PMA (100 nM)– and Ang II (1 μM)–dependent O2- and ROS production was analyzed after incubating the cells with Irb for 5 minutes at 37°C (C,D). Chemiluminescence intensity was recorded in a Wallac Victor2 (Perkin Elmer) apparatus for 10 minutes. Data are expressed as the mean ± SEM of 3 experiments.

Angiotensin II enhances O2- and ROS production in a time- and dose-dependent manner in human neutrophils. Inhibitory effect of irbesartan. Neutrophils were suspended in KR-HEPES buffer at 106 cells/mL in the presence of 15 μM lucigenin (A,C) or 15 μM luminol (B,D). Reactions were started by the addition of angiotensin II (Ang) at the indicated concentrations. The effect of irbesartan (Irb, 10 μg/mL) on PMA (100 nM)– and Ang II (1 μM)–dependent O2- and ROS production was analyzed after incubating the cells with Irb for 5 minutes at 37°C (C,D). Chemiluminescence intensity was recorded in a Wallac Victor2  (Perkin Elmer) apparatus for 10 minutes. Data are expressed as the mean ± SEM of 3 experiments.

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