Figure 3.
Figure 3. PD098059 and SB203580 inhibit angiotensin II–dependent O2- and ROS production in human neutrophils. Neutrophils were suspended in KR-HEPES buffer at 106 cells/mL and were incubated with PD098059 (50 μM) or SB203580 (50 μM) for 30 minutes. Lucigenin (15 μM) (A,C) or luminol (15 μM) (B,D) was added, and the reactions were started by the addition of angiotensin II (Ang) at the indicated concentrations. Chemiluminescence intensity was recorded in BioOrbit 1252 luminometer for the indicated times. Data are expressed as the mean ± SEM of 3 experiments.

PD098059 and SB203580 inhibit angiotensin II–dependent O2- and ROS production in human neutrophils. Neutrophils were suspended in KR-HEPES buffer at 106 cells/mL and were incubated with PD098059 (50 μM) or SB203580 (50 μM) for 30 minutes. Lucigenin (15 μM) (A,C) or luminol (15 μM) (B,D) was added, and the reactions were started by the addition of angiotensin II (Ang) at the indicated concentrations. Chemiluminescence intensity was recorded in BioOrbit 1252 luminometer for the indicated times. Data are expressed as the mean ± SEM of 3 experiments.

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