TfR1/Actin × 10² and Hepc/Actin ratios in HepG2 cells after incubation with 50 μM deferoxamine, 2.5 g/L iron saturated diferric transferrin (equivalent to 65 μM transferrin-bound Fe[III]), and 65 μM Fe-NTA (equivalent to 65 μM non-transferrin-bound Fe[III]) for 24 hours. The results (mean values ± 95% confidence intervals) of 8 independent experiments are shown. (A) The increase of the TfR1/Actin × 10² ratio after incubation with 50 μM deferoxamine and the decrease of the TfR1/Actin × 10² ratio after incubation with 2.5 g/L iron-saturated diferric transferrin and 65 μM Fe-NTA was statistically significant (P < .01). (B) The decrease of the Hepc/Actin ratio after incubation with 65 μM Fe-NTA was also statistically significant (P < .001).