Flow cytometric analysis of 7/4⁺Gr-1^hi and 7/4⁺Gr-1^int bone marrow populations. (A) Flow cytometric analysis of 7/4/Gr-1, 7/4/NIMP-R14 and Mac-1/Gr-1 staining of peritoneal lavage cells 4 hours after TG injection. Gr-1^hi neutrophils, R1; 7/4⁺Gr-1^int or NIMP-R14^int cells, R2; Mac-1⁺Gr-1^lo monocyte/macrophages, R3. (B) Histograms of 7/4⁺Gr-1^hi (solid line), 7/4⁺Gr-1^int (gray fill), and isotype control (dotted line) are shown. The 7/4⁺Gr-1^int profiles were similar in cells from blood, spleen, and peritoneal lavage (4 hours after TG).