American Society of Hematology

Figure 5.

$Figure 5. Subcellular distribution of radiolabeled sTNFR1-tm-Y, and endogenous RMCP-II. RBL cells were biosynthetically radiolabeled for 0.5 hours (pulse), followed by chase of the label for 2 hours (chase). At the pulse and chase intervals, 100 × 106 cells were removed and homogenized, and the postnuclear supernatant was subcellular fractionated by centrifugation in Percoll. The fractions were immunoprecipitated with anti-sTNFR1 (A) or anti–RMCP-II (B) and analyzed as described in “Materials and methods.” The densitometry data are shown for pulse (□) and chase (##) experiments as percent of total mature and processed forms of sTNFR1-tm-Y or RMCP-II. The position of sTNFR1-tm-Y and RMCP-II are indicated with arrows, and the various processed forms are indicated with dotted lines. Molecular weight markers are shown to the left in kDa.$

Subcellular distribution of radiolabeled sTNFR1-tm-Y, and endogenous RMCP-II. RBL cells were biosynthetically radiolabeled for 0.5 hours (pulse), followed by chase of the label for 2 hours (chase). At the pulse and chase intervals, 100 × 10⁶ cells were removed and homogenized, and the postnuclear supernatant was subcellular fractionated by centrifugation in Percoll. The fractions were immunoprecipitated with anti-sTNFR1 (A) or anti–RMCP-II (B) and analyzed as described in “Materials and methods.” The densitometry data are shown for pulse (□) and chase (##) experiments as percent of total mature and processed forms of sTNFR1-tm-Y or RMCP-II. The position of sTNFR1-tm-Y and RMCP-II are indicated with arrows, and the various processed forms are indicated with dotted lines. Molecular weight markers are shown to the left in kDa.

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