α-Granular protein digestion. (A) Western blot of platelet lysate (2.5 μg per lane) from line no. 13 and no. 19 and wild-type mice immunoblotted with a rabbit antihuman VWF polyclonal antibody. (B) Western blot of nonreduced gel of platelet lysates as above or plasma (3 μg per lane for mouse and 6 μg per lane for human) proteins. H indicates human plasma preparation. Murine and human fibrinogen were detected using a goat antihuman fibrinogen polyclonal antibody. (C) Same as panel B except that the samples were only of platelet lysates and the gel was performed under reducing conditions.