Efficient engraftment and complete reconstitution of human lymphocytes in NOD/SCID/γ_c^null mice that underwent transplantation with CB CD34⁺ cells. 1 × 10⁴ (n = 7) or 5 × 10⁴ (n = 17) CB CD34⁺ cells were transplanted into NOD/SCID/γ_c^null mice, and 1 × 10⁵ (n = 15) CB CD34⁺ cells were transplanted into NOD/SCID mice. (A) Percentages of human CD45⁺cells in PB of mice were analyzed 3 months after transplantation using flow cytometry. Percentages of human CD45⁺cells were calculated as: % human CD45⁺ = human CD45⁺cells/(human CD45⁺cells ⁺mouse CD45⁺cells) × 100. Results of 7 independent experiments are shown. (B) Representative FACS analysis of human lymphocytes in BM and spleen of NOD/SCID/γ_c^null mice that underwent transplantation with CB CD34⁺ cells. (C) Representative FACS analysis of KIR antigens is shown. CD158a or KIR p70 were detected on 7.7% or 3.8% of CD56⁺cells, respectively.