Eotaxin-3 acts as an antagonist on CCR2-transfected cells. (A) MCP-1 induced chemotaxis of CCR2-transfected cells in the presence (▴) or absence (•) of eotaxin-3 at 1 μM. Cell migration was evaluated in Boyden microchambers. All determinations were performed in triplicate. The control indicates the number of cells migrating in medium alone. (B) Chemotaxis of CCR2-transfected cells was evaluated in 24-transwell chambers. Where indicated, eotaxin-3 (1 μM) was applied to the cells in the upper well, and 10 nM MCP-1–induced migration was assessed. Data are expressed as chemotaxis index.