Results after viral and clinical relapse. (A) ALT values, serum HCV RNA titers, BL HCV RNA detection, and t(14;18) translocation in a representative patient (no. 3) who had a relapse during follow-up after a transient CR. (B) DNA sequence of bcl-2/J_H junctions detected in total DNA extracted from peripheral BLs of the same patient, taken at time 0 (pretreatment) and at 9 months of follow-up (posttreatment); bcl-2, N-segment, J_H: sequences of the bcl-2/J_H junction corresponding to bcl-2 gene (chromosome 18), N-segment, and J_H region of the IgH locus (chromosome 14), respectively. Line bcl-2/J_H: alignment was done on bcl-2 (GenBank accession no. M14745) and IgH locus J-regions (accession no. M25625; line bcl-2/J_H). Pt indicates patient number (Tables 1 and 2); pre and post, sequences corresponding to peripheral blood samples taken before and after IFNα treatment, respectively. Dots indicate identical nucleotides; gaps in sequences (–) were introduced for clarity.