Induction of apoptosis by NaAsO₂ correlates with the inhibition of NF-κB.(A) The indicated cell lines were incubated for 24 hours with PBS (□)or 2(▦), 5 (▨), and 10 (▪) μM NaAsO₂. The percentage of apoptotic cells was determined by FACS analysis of annexin V⁺ cells. Error bars denote SDs. (B) The cell lines HDLM-2, L428, and KMH-2 were treated for 24 hours with the indicated concentrations of NaAsO₂. Whole cell extracts were analyzed by EMSA for NF-κB DNA binding activity. Free DNA probe is not shown; n.s. indicates nonspecific.