Successful incorporation of antibody into platelets by DMSO treatment. Confocal images of washed platelets loaded with UN7.79 using DMSO as described in “Materials and methods.” Platelets were allowed to spread for 45 minutes, then fixed and stained with secondary antibody to detect UN7.79 (red) or phalloidin to detect F-actin (green). Although filopodia are visible, CIB cannot be detected at the tip of the filopodia owing to antibody inhibition of CIB relocation. Original magnification, × 600.