Effect of incorporation of CIB protein and GPIIb peptide. Incorporation of CIB protein along with GPIIb peptide allows platelets to spread. (A) Confocal images of washed platelets allowed to spread on Fg for 45 minutes and stained for F-actin. Platelets loaded with GPIIb cytoplasmic peptide (GPIIb Pep) showed long filopodia. Platelets loaded with GPIIb peptide and recombinant CIB protein (GPIIb Pep + CIB) recovered to a spread platelet morphology. Original magnification, × 600. (B) Quantitation of the percentage of spread platelets in panel A. Error bars indicate mean ± sem of at least 3 independent experiments.