IgE alone stimulates the adhesion of both BMMCs and CTMCs to FN. (A) Adhesion of normal BMMCs to FN following a 60-minute adhesion assay with increasing concentrations of IgE alone (SPE-7, top left panel; Liu anti-DNP or anti-Epo26, top right panel), SF (bottom left panel), or IgE + DNP-HSA (bottom right panel). The black bars in the top left panel indicate the level of adhesion to wells coated with BSA instead of FN. (B) A time course of BMMC adhesion to FN in the presence of 1 μg/mL IgE alone (•), 5 ng/mL SF (▵), or IgE + 2 ng/mL DNP-HSA (□). Background adhesion (2%-6%) was subtracted from each time point. (C) Adhesion of normal CTMCs to FN in response to IgE at the indicated concentrations, 5 ng/mL SF, or IgE + 5 ng/mL DNP-HSA for 60 minutes. Results shown are the mean ± SEM of triplicate determinations. Similar results were obtained in 5 (A), 3 (B), and 2 (C) separate experiments. Adhesion in response to all stimuli was significantly (P < .05) different from control values except for (C) 0.1 μg/mL.