SHIP-2 localizes to actin-rich regions in spreading platelets. (A) Platelets spread on a VWF matrix were fixed, permeabilized, stained with SHIP-2 antibodies, or costained with affinity-purified preimmune sera and phalloidin and visualized by confocal microscopy. Arrows indicate SHIP-2 staining at filopodia (ii), the central actin ring (iii), lamellipodia (iv) and diffuse central staining detected in some spread platelets (v). Bar, 10 μm. (B) Platelets spread on a VWF matrix were fixed, permeabilized, costained with affinity-purified SHIP-2 antibodies and phalloidin, and visualized by confocal microscopy. Arrows indicate colocalization of SHIP-2 and phalloidin at filopodia (i-ii), at the inner actin ring (iii) and at lamellipodia (iv) (merged images). The arrow showing SHIP-2 staining in subpanel iv indicates diffuse central staining detected in some spread platelets. Bar, 10 μm.