Direct intrafemoral injection of recombinant adenovirus. The rAdluc virus was produced, purified, and administered by DII according to the procedures described in “Materials and methods.” Following DII, BMCs were harvested from individual mice (1-6) and assayed for luciferase activity 24 hours after culture in vitro (A) or were harvested from mice 24 hours after DII in vivo (B). Saline injection was used as a negative control (-), and DII of femurs removed from host was used as a positive control (+). These results are representative of 3 separate experiments.