TG-GFP⁺ and KI-GFP⁺ cells in the AGM region express CD34 and PCLP1 antigens. (A) TG-GFP expression in the AGM region of E11.5 embryo (line no. 685). The cells in the region between 2 yellow lines were analyzed. Original magnification, × 50. (B-C) Flow cytometric analysis of 3.1ISIGII GFP transgenic embryos (line no. 685, B) and KI-heterozygous embryos (C).