The function of GATA-2 is domain-specific in primary cultures of AGM cells. (A) Schematic representation of the GATA-2 domain mutants integrated into the genome of MSCV-IRES-EGFP virus. (B) Western blot analysis of infected LO cells with anti–GATA-2 antibodies, C-20 and RC.1.1.1, and anti-GFP antibody. Note comparable expression of each mutant protein. (C) Numbers of CD45⁺ cells in cultures of AGM cells infected with each virus. Results are shown relative to control cultures. Each point and bar represents the mean ± standard deviation of 3 independent experiments. The results of del236 and VP-F are 5.21 ± 3.33 and 1.99 ± 0.72, respectively; and del236 and VP-F resulted in significantly higher numbers of CD45⁺ cells than wild-type GATA-2 (P < .05, Student t test). The significance of the differences was confirmed by repeated experiments (wild-type del 236, n = 12, P < .0001; wild-type VP, n = 7, P < .0001; Student t test).