Sig-α₂ peptide increases collagen binding to resting platelets. Human platelets were incubated with 200 μM Sig-α₂ peptide or Sig-scrambled α₂ peptide at room temperature for 30 minutes before a 2-minute treatment with control buffer or 50 μM TRAP. Platelets were then fixed with PFA, washed, incubated with 25 μg/mL FITC-collagen for 30 minutes, and evaluated by flow cytometry. Data are shown as averaged normalized percent fluorescence over basal binding in the absence of peptide (set to zero) ± SEM from 5 independent experiments. *P < .05 and ⁽*⁾P > .5 compared with no peptide control; ** P > .1 (ie, not significantly different) compared with Sig-α₂ scramble peptide in TRAP-stimulated group; ⁽**⁾P > .5 (not significantly different) versus TRAP-stimulated control.