FITC-collagen binding induced by the Sig-KLGFFKR peptide is largely surface associated. Human platelets were incubated with control buffer or the Sig-KLGFFKR peptide at a final concentration of 200 μM and exposed to 25 μg/mL FITC-collagen. The reactions were stopped by the addition of 10 × ice-cold PBS at the indicated time points. For quenched groups, trypan blue was added at a final concentration of 200 μg/mL. Data shown are the normalized mean fluorescence ± SEM from 2 independent experiments, representing 3 similar experiments.