Src kinases and PI3-kinase support limited activation of PLCγ2-deficient platelets via GPVI. (A) Washed platelets from PLCγ2^–/– mice were incubated with DMSO, 30 μM PP1, or 100 nM wortmannin for 5 minutes and then stimulated with 20 μg/mL collagen for 5 minutes. (B) Washed platelets from PLCγ2^–/– mice were stimulated with 10 μg/mL CRP for 5 minutes in the presence of fibrinogen (200 μg/mL) and the inhibitors described for panel A. (C) Washed platelets from PLCγ2^–/– were stimulated with 40 μg/mL convulxin for 5 minutes under the same conditions as described for panel B. The data are representative of at least 2 experiments.