American Society of Hematology

Figure 2.

$Figure 2. p21-antisense reduces the G0 fraction of transduced CD34+ cord blood cell population. (A) Histogram of cellular RNA staining by Pyronin Y (PY) in the HoechstlowCD34+38– cell fraction of CD34+ cord blood cells transduced with a lentiviral vector containing p21-antisense (red line) or with the control vector (blue line) 4 days after beginning of transduction. PYlow cells represent cells in G0. Chart shows a representative experiment. (B) Mean ± SEM of CD34+38– cells in G0 after transduction of CD34+ cord blood cells with p21-AS-V or the control vector, GFP (n = 6).$

p21-antisense reduces the G₀ fraction of transduced CD34⁺ cord blood cell population. (A) Histogram of cellular RNA staining by Pyronin Y (PY) in the Hoechst^lowCD34⁺38^– cell fraction of CD34⁺ cord blood cells transduced with a lentiviral vector containing p21-antisense (red line) or with the control vector (blue line) 4 days after beginning of transduction. PY^low cells represent cells in G₀. Chart shows a representative experiment. (B) Mean ± SEM of CD34⁺38^– cells in G₀ after transduction of CD34⁺ cord blood cells with p21-AS-V or the control vector, GFP (n = 6).

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