HA induces FGF-2 secretion by HCs. HCs were cultured for 24 hours at 37°C in 24-well plates coated with HA; control substrata were BSA-coated or uncoated plastic (PL). FGF-2 was measured by a commercial ELISA. A culture time of 24 hours was chosen because we have previously shown that, during this period of culture, HA induces substantial FN production by HCs. The results represent the means of triplicate measurements involving the cells of 4 different HCL patients.