Figure 6.
Figure 6. Determination of the functional stability over time at 37°C of the recombinant FV variants. (A) Recombinant FV variants (0.5 nM) were incubated at 37°C over time. Subsamples were withdrawn at different time points and activated with thrombin (0.5 U/mL) for 10 minutes, and the remaining procoagulant activity was then determined with the prothrombinase assay. (B) The recombinant FV variants (0.5 nM) were activated with thrombin (0.5 U/mL) for 10 minutes and then left on 37°C. Subsamples were then withdrawn at different time points, and the remaining activity was determined as in panel A. WT FVa (▪), Ala221Val (□), Ala221Gly (▴), Glu275Gln (▵), and Cys220Ala/Cys301Ala (•). Each data point represents the mean of 3 independent experiments performed in duplicate. Error bars represent ± SDs.

Determination of the functional stability over time at 37°C of the recombinant FV variants. (A) Recombinant FV variants (0.5 nM) were incubated at 37°C over time. Subsamples were withdrawn at different time points and activated with thrombin (0.5 U/mL) for 10 minutes, and the remaining procoagulant activity was then determined with the prothrombinase assay. (B) The recombinant FV variants (0.5 nM) were activated with thrombin (0.5 U/mL) for 10 minutes and then left on 37°C. Subsamples were then withdrawn at different time points, and the remaining activity was determined as in panel A. WT FVa (▪), Ala221Val (□), Ala221Gly (▴), Glu275Gln (▵), and Cys220Ala/Cys301Ala (•). Each data point represents the mean of 3 independent experiments performed in duplicate. Error bars represent ± SDs.

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