American Society of Hematology

Figure 1.

$Figure 1. Analysis of lacZ expression in thymocytes from Sp3+/- embryos by 4-color cytometry. Thymus cell suspensions were loaded with FDG substrate and subseqently stained for CD3, CD4, and CD8. (A) Total thymocytes were analyzed for CD3 expression and lacZ activity. (B) Cells were analyzed for CD3 expression; CD3- and CD3+ fractions were analyzed for CD4 and CD8; and the indicated subpopulations were gated and analyzed for lacZ activity. The results are displayed as histograms of Sp3+/- embryos (bold lines), together with those of wild-type embryos (dashed lines). The numbers are the proportions of lacZ-expressing cells (mean values ± SD, n = 6). Background values in wild-type mice (n = 4) were below 1% in all fractions, except for the CD3+ double-dull (DD) fraction, in which 3% ± 1% of the cells were lacZ+.$

Analysis of lacZ expression in thymocytes from Sp3^+/- embryos by 4-color cytometry. Thymus cell suspensions were loaded with FDG substrate and subseqently stained for CD3, CD4, and CD8. (A) Total thymocytes were analyzed for CD3 expression and lacZ activity. (B) Cells were analyzed for CD3 expression; CD3^- and CD3⁺ fractions were analyzed for CD4 and CD8; and the indicated subpopulations were gated and analyzed for lacZ activity. The results are displayed as histograms of Sp3^+/- embryos (bold lines), together with those of wild-type embryos (dashed lines). The numbers are the proportions of lacZ-expressing cells (mean values ± SD, n = 6). Background values in wild-type mice (n = 4) were below 1% in all fractions, except for the CD3⁺ double-dull (DD) fraction, in which 3% ± 1% of the cells were lacZ⁺.

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