Figure 1.
Induction of CTLs in mice immunized with DCs transfected with VEGF and VEGFR-2 mRNA. DCs generated from C57BL/6 mice were electroporated with VEGF (▪), VEGFR-2 (▴), or actin (□) mRNA, and naive, syngeneic mice were immunized with 5 × 105 cells 3 times intravenously. Splenocytes were harvested, restimulated with DCs transfected with RNA (same as used in the immunization) for 5 days, and tested for the presence of CTLs. BLK.SV cells (BL/6 fibroblasts transformed with SV40) transfected with mRNA as indicated were used as targets. Data are expressed as mean percent specific lysis of triplicate samples ± SD.
