Figure 4.
Failure of sFasL to desensitize neutrophils to the subsequent stimulation by FMLP. Locomotory activity of neutrophils incubated in the upper compartment of a chemotaxis chamber with medium and exposed in the lower compartment for 45 minutes to medium (Nil), 10 nM FMLP (FMLP), or 0.1 nM sFasL was compared with locomotory activity of cells incubated in the upper compartment with 10 nM sFasL (sFasL + FMLP) or RBC supernatant (1:1 × 105, vol/vol dilution, SUP-RBCs + FMLP) and exposed in the lower compartment to 10 nM FMLP. Results are expressed as mean ± 1 SD of 3 experiments with neutrophils from different donors.
