Figure 5.
Desensitization of neutrophil response to FMLP by TGF-β1. Locomotory activity of neutrophils incubated in the upper compartment of a chemotaxis chamber with medium and exposed in the lower compartment for 45 minutes to medium (Nil), 10 nM FMLP (FMLP), or 40 fM TGF-β1 was compared with locomotory activity of cells incubated in the upper compartment with 40 fM TGF-β1 (TGF-β1 + FMLP) or RBC supernatant (1:1 × 105, vol/vol dilution, SUP-RBCs + FMLP) and exposed in the lower compartment to 10 nM FMLP. Results are expressed as mean ± 1 SD of 3 experiments with neutrophils from different donors.
