Figure 9.
Figure 9. Effect of RBC supernatant on the FMLP-induced polarization and redistribution of CD11b. (A) Neutrophils exposed to FITC-conjugated anti-CD11b mAb. (B) Neutrophils exposed to 1 μM FMLP and labeled with FITC-conjugated anti-CD11b mAb. (C) Neutrophils preincubated with RBC supernatant (1:1 × 105, vol/vol dilution), exposed to 1 μM FMLP, and labeled with FITC-conjugated anti-CD11b mAb. (D) Neutrophils preincubated with TGF-β1–depleted RBC supernatant (1:1 × 105, vol/vol dilution), exposed to 1 μM FMLP, and labeled with FITC-conjugated anti-CD11b mAb.

Effect of RBC supernatant on the FMLP-induced polarization and redistribution of CD11b. (A) Neutrophils exposed to FITC-conjugated anti-CD11b mAb. (B) Neutrophils exposed to 1 μM FMLP and labeled with FITC-conjugated anti-CD11b mAb. (C) Neutrophils preincubated with RBC supernatant (1:1 × 105, vol/vol dilution), exposed to 1 μM FMLP, and labeled with FITC-conjugated anti-CD11b mAb. (D) Neutrophils preincubated with TGF-β1–depleted RBC supernatant (1:1 × 105, vol/vol dilution), exposed to 1 μM FMLP, and labeled with FITC-conjugated anti-CD11b mAb.

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