CD8⁺ T-cell subsets in controls and patients with B-CLL. Representative dot plot of a 3-color immunostaining of CD8⁺ T-cell subsets in a healthy control (left) and a patient with Rai stage 0 B-CLL (right). Cells were gated on lymphogate via forward/side scatter (FSC/SSC) and subsequently on CD8-PercP after which the following subsets can be distinguished based on CD45RA-PE and CD27-FITC: CD45RA⁺CD27^- cytotoxic effector cells, CD45RA⁺CD27⁺ naive cells, and CD45RA^-CD27⁺ noncytotoxic cells.