Quantification of gene expression by real-time RT-PCR. Quantitative real-time RT-PCR of 12 genes was performed after 4 hours and 24 hours of incubation with Stxs. Data shown were obtained from 2 independent experiments. CSF2 indicates colony-stimulating factor 2 (granulocyte-macrophage); EGR-1, early growth response 1; GRO-1, GRO-1 oncogene; GRO-2, GRO-2 oncogene; GRO-3, GRO-3 oncogene; ICAM1, intercellular adhesion molecule-1; IL-6, interleukin 6; IL-8, interleukin 8; LAMB3, laminin beta 3; MCP-1, monocyte chemotactic protein 1; NFκB2, nuclear factor of kappa light polypeptide gene enhancer in B cells 2; and TRAF-1, TNF receptor–associated factor 1.