Figure 3.
Expression of TEL/PDGFβR protects BaF/3 from apoptosis following IL-3 withdrawal. BaF/3 clone TP2 was incubated in the presence (+ Tet) or absence (– Tet) of 2 μg/mL Tet for 24 hours, washed, and incubated for a further 24 or 48 hours (+ or – Tet) with no IL-3. Cells were stained for 15 minutes with 10 nM Di0C6 and 10 000 events were analyzed per sample by FACS on FL1 channel.
