Inhibition of ERK and JNK MAPKs partially protects cells from apoptosis induced by the combination of IL-3 and TEL/PDGFβR signals. Cells were incubated in the presence (+ Tet) or absence (– Tet) of 2 μg/mL Tet for 24 hours, washed, and incubated for a further 24 or 48 hours (+ or – Tet) with (A) 20 pg/mL IL-3, (B) 20 pg/mL IL-3 with 10 μM U0126, or (C) 20 pg/mL IL-3 with 5 μM SP600125. Cells were stained for 15 minutes with 10 nM Di0C6 and 10 000 events analyzed per sample by FACS on channel FL1. (D) Bar chart summarizes the effects of TEL/PDGFβR expression on IL-3–induced survival in the presence of U0126 and SP600125. Data from 4 independent experiments using clones TP2 and TP15 were used. Mean and SEM are plotted for each treatment and 2-tailed paired Student t tests were applied to the data and values of significance indicated (P).
