Intraclonal heterogeneity of PIM-1 sequences in AIDS-DLBCL 2378. Presumed genealogic tree showing the relationship between PIM-1 mutations identified in the neoplastic population from AIDS-DLBCL case 2378. Sixty-five clones, obtained from 2 independent PCR amplifications, were sequenced. Each circle represents a distinct sequence carrying the mutations detailed on the side (GenBank accession no. AF386792); the number of independent clones sharing those mutations is indicated within the circle. Alleles A and B were defined according to the presence and distribution of the mutations that had been detected by direct sequencing and, therefore, represent the original, most abundant tumor clone (see also Table 4). The dashed circle indicates presumed intermediates in the genealogic tree.