Enhancement of RCBX64 transcriptional activity by double-stranded oligonucleotides containing the 12-bp motif. A single copy of each double-stranded oligonucleotide (Table 1) was subcloned into the BglII site of RCBX64 in the forward direction as indicated below the figure. The transcriptional activity of each plasmid was determined in H4IIEC3 cells. The activity is shown as the average fold-stimulation relative to RCBX64 without an oligonucleotide (–), n = 3. Error bars indicate SD. (A) WT and 3-bp mutants A through D. (B) WT and 2-bp mutants E through J.