Figure 2.
![Figure 2. Biospecific interaction analysis in real time between p97 and various anti-p97 mAbs. p97 was immobilized on a sensor chip (CM5) using standard coupling procedures incorporating NHS, EDC, and ethanolamine. Different mAbs directed against p97 (HybC, HybE, HybF, L235, 2C7, 9B6), diluted to 0.05 μg/μL in Ringer-HEPES, were injected into the BIAcore at a flow rate of 5 μL/min. The surface plasmon resonance response obtained for these mAbs was plotted (in relative units [RU]) as a function of time. After each injection, immobilized p97 was regenerated with 0.2M glycine at pH 2 for 2 minutes followed by a 2-minute injection of ferric ammonium citrate (1 mM) (n = 4).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/5/10.1182_blood-2003-01-0166/6/h81734874002.jpeg?Expires=1735580579&Signature=MwEScDp1wSbrjak56SfiFCNn~6Jl1AuJ-C2X-A2fryR6yTljxd~a-Yq27JV-jWZ~dY~qbLVnUYVM-PJ50-BSP-zQ5C7gwei~7HDzcUJlEIyS-FzY4vtgrG6Nl-MKLPxLsY3iHlFmvgTagQvsQcpyztIEzQPwAMtzX3vLFhmxQU~WoiMmrfkecmGE~-uxmckm3sAkBLEC7EbuNzcQ5xxUvEDZu0Ac54f13b-BzuTcBOAFDINU7nVE3n9dPxFOdT97ShoIveBlDjJ0TvL8qB0ICX8LPt~JgYLLnXCK4WkZsHw8nBLY5quE-8xcHaBvN4BaCU4VfUrQqro9x294usWAIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Biospecific interaction analysis in real time between p97 and various anti-p97 mAbs. p97 was immobilized on a sensor chip (CM5) using standard coupling procedures incorporating NHS, EDC, and ethanolamine. Different mAbs directed against p97 (HybC, HybE, HybF, L235, 2C7, 9B6), diluted to 0.05 μg/μL in Ringer-HEPES, were injected into the BIAcore at a flow rate of 5 μL/min. The surface plasmon resonance response obtained for these mAbs was plotted (in relative units [RU]) as a function of time. After each injection, immobilized p97 was regenerated with 0.2M glycine at pH 2 for 2 minutes followed by a 2-minute injection of ferric ammonium citrate (1 mM) (n = 4).
