Molecular interactions of p97 and various components of the PA:plasmin system. (A) Pro-uPA and tPA (0.05 μg/μL), diluted in Ringer-HEPES, were injected onto immobilized p97 on a sensor chip at a flow rate of 5 μL/min. The SPR response for these proteins was plotted in RU as a function of time. p97 (0.05 μg/μL) was also injected over immobilized PAI-1 (p97/PAI-1). (B) Plasminogen, plasmin, or angiostatin (0.05 μg/μL) was injected onto immobilized p97. The SPR response for these proteins was plotted in RU as a function of time. The results indicate that pro-uPA and plasminogen interact with p97. After each injection the sensor chip surface with immobilized p97 was regenerated by injecting 10 mM glycine, pH 2.2, for 2 minutes.