Effect of p97 on plasminolytic activity induced by pro-uPA. (A) The plasminolytic activity of 1 nM pro-uPA was measured without (○) or with (•) 70 nM p97 in the presence of 30 nM plasminogen. The reaction was performed in a final volume of 200 μL as described in “Materials and methods.” As a control, the enzymatic activity in the presence of p97 alone was also measured (▪). (B) Plasmin activity induced by pro-uPA was measured in the presence of various p97 concentrations. (C) Plasmin activity induced by pro-uPA was measured without (○) or with (•) 250 nM p97 and various concentrations of plasminogen. (D) Inhibition by the mAb L235 of the increase in plasminolytic activity induced by p97. The plasminolytic activity of pro-uPA was measured in the presence of 70 nM p97 and 65 nM of either mAb L235 (○) or nonspecific mouse IgG (•). One representative experiment is shown and data represent the means ± SDs (n = 3)