Figure 4.
Constitutively active Ras induces FucT-VII expression in JSB3 cells. JSB3 cells were infected with either control or V-H-Ras retrovirus and analyzed 3 days after infection. (A) GFP expression marks virally infected cells and gives a quantitative measure of the level of expressed Ras on a per cell basis. HECA-452 staining is induced selectively on Ras/GFP-high cells; Ras/GFP+ cells remain HECA-452–. (B) Inhibition of Ras-induced FucT-VII expression by MAPK inhibitors. JSB3 cells were infected with Ras retrovirus, and inhibitors were included at the time of infection. Inhibitors are exactly as in Figure 1. *P < .05 versus treatment with no inhibitors; **P < .05 versus groups with a single asterisk (*).