Constitutively active Raf induces FucT-VII expression in JSB3 cells. JSB3 cells were infected with either control or Raf-BXB retrovirus and analyzed 3 days after infection. (A) HECA-452 staining is induced selectively on Raf-BXB/GFP-high cells; Raf-BXB/GFP⁺ cells remain HECA-452^–. Also note lower fraction of cells that express HECA-452 epitopes than in cells expressing active Ras. (B) Inhibition of Raf-BXB–induced FucT-VII expression by MAPK inhibitors. JSB3 cells were infected with Raf-BXB retrovirus, and inhibitors were included at the time of infection. Inhibitors are as in Figures 2 and 4. *P < .05 versus treatment with no inhibitors.